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KMID : 0604520110370040319
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Journal of the Society of Cosmetic Scientists of Korea
2011 Volume.37 No. 4 p.319 ~ p.326
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Effect of Nigella sativa Oil on Melanogenesis
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Lee Su-Yeon
Lee Sae-Mi
Heo Woo-Beom
Kim Jin-Guk
Kim Young-Heui
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Abstract
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In order to investigate the potential of Nigella sativa (N. sativa) oil as an active ingredient for whitening cosmetics, we prepared N. sativa oil. We measured its inhibitory effects on mushroom tyrosinase activity, cellular tyrosinase activity, and melanin synthesis inhibitory activity in B16 melanoma cells. N. sativa oil and its components showed inhibitory activity against mushroom tyrosinase and melanin synthesis. In a melanin synthesis inhibition assay using mouse B16-F10 melanoma cell, it reduced melanin production up to 86 % at a concentration of 10 mg/mL without cytotoxicity. In the study on the melanogenic protein expressions by using RT-PCR and Western blot, N. sativa oil and its components inhibited expression of tyrosinase protein, which is a well-known key protein on melanogenesis, and tyrosinase expression was gradually decreased in a dose-dependent manner. Therefore, this result suggests that N. sativa oil could be used as an active ingredient for whitening cosmetics.
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KEYWORD
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skin-whitening, Nigella sativa, thymoquinone, thymohydroquinone, B16 melanoma
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